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Group IV (Control-combination) was administered PBS for 3 days, then orally given a combination therapy of GABA (200 mg/kg/day for 21 days), probiotics (Fiber probiotic, Proflora, 0.2 g/kg/day for 21 days), vitamin D3 (SUN-D 1000, California Greens, 1,000 IU/kg/day for 14 days) and β -lactam (Amoxicillin, HYMOX®, 200 mg/kg/day for 7 days) as described in previous studies (Bin-Khattaf et al., 2022

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NMR chemical shift perturbation analysis The CSPs were calculated according to the following equation: where Δ δH and Δ δN indicate the chemical shift changes of the amide proton and nitrogen, respectively, using CcpNMR 2.5 49